Rui Miguel Pinheiro Vitorino
  Telefone:
  Extensão: 
23507
  e-mail:
rvitorino@ua.pt
  Categoria: 
Investigador auxiliar convidado
  Departamento:
Química
  Gabinete:
15.1.21
  Grupo de investigação:
Espectrometria de Massa
  Pesquisa no ISI web of knowledge:
Vitorino R
  Saber mais em:
 




Formação académica


2004: Doutoramento em Bioquímica, Universidade de Aveiro

2000: Licenciatura em Química- Ramo da Bioquímica e Química Alimentar, Universidade de Aveiro



Percurso profissional

Desde 2008: Investigador auxiliar convidado, Departamento de Química da Universidade de Aveiro


Interesses científicos

Proteómica e peptidómica da saliva em condições patofisiológicas.

Desenvolvimento de sistemas de enriquecimento em nano-escala para caraterização de modificações pós-traducionais.



Grupo de investigação

Pós-Doutoramento

José Alexandre Ferreira. “The role of mitochondria in the energy metabolism of tumor cells: a proteome perspective” SFRH/BPD/66288/2009


Doutoramento

Virginia Maria Dinis Carvalhais. Effect of modulating S. epidermidis biofilms dormancy in the biofilm physiology and host immune response. Programa Doutoral em Ciências Biomédicas, ICBAS Universidade do Porto, SFRH/BD/78235/2011, desde Janeiro 2012.




Projetos em curso

Desenvolvimento de uma plataforma em nanoescala para o enriquecimento de proteases EXPL/BBB-BEP/0317/2012, Investigador principal.


Avaliação bioquímica, morfológica e funcional do catabolismo muscular associado ao cancro da mama: o papel do exercício físico, PTDC/DES/114122/2009, membro de equipa.



Artigos do SCI

  1. Analysis of low abundance membrane-associated proteins from rat pancreatic zymogen granules. Borta H, Aroso M, Rinn C, Gomez-Lazaro M, Vitorino R, Zeuschner D, Grabenbauer M, Amado F, Schrader M. J Proteome Res. 2010; 1;9(10):4927-39.

  2. Finding new posttranslational modifications in salivary proline-rich proteins. Vitorino R, Alves R, Barros A, Caseiro A, Ferreira R, Lobo MC, Bastos A, Duarte J, Carvalho D, Santos LL, Amado F. Proteomics. 2010; 10(20):3732-42.

  3. Genomic and proteomic evaluation of antibiotic resistance in Salmonella strains. Pinto L, Poeta P, Vieira S, Caleja C, Radhouani H, Carvalho C, Vieira-Pinto M, Themudo P, Torres C, Vitorino R, Domingues P, Igrejas G. J Proteomics. 2010; 73(8):1535-41.

  4. Identification of salivary proteins at oil-water interfaces stabilized by lysozyme and beta-lactoglobulin. Silletti E, Vitorino R, Schipper R, Amado FM, Vingerhoeds MH. Arch Oral Biol. 2010 55(4):268-78.

  5. Lifelong physical activity modulation of the skeletal muscle mitochondrial proteome in mice. Alves RM, Vitorino R, Figueiredo P, Duarte JA, Ferreira R, Amado F. J Gerontol A Biol Sci Med Sci. 2010; 65(8):832-42.

  6. Oxidative modifications in glycated insulin. Guedes S, Vitorino R, Domingues MR, Amado F, Domingues P. Anal Bioanal Chem. 2010; 397(5):1985-95.

  7. Proteomic characterization of vanA-containing Enterococcus recovered from Seagulls at the Berlengas Natural Reserve, W Portugal. Radhouani H, Poeta P, Pinto L, Miranda J, Coelho C, Carvalho C, Rodrigues J, López M, Torres C, Vitorino R, Domingues P, Igrejas G. Proteome Sci. 2010 Sep 21;8:48.

  8. Salivary peptidomics. Amado F, Lobo MJ, Domingues P, Duarte JA, Vitorino R. Expert Rev Proteomics. 2010 Oct;7(5):709-21.

  9. Subsarcolemmal and intermyofibrillar mitochondria proteome differences disclose functional specializations in skeletal muscle. Ferreira R, Vitorino R, Alves RM, Appell HJ, Powers SK, Duarte JA, Amado F. Proteomics. 2010; 10(17):3142-54.

  10. Characterization of mitochondrial proteome in a severe case of ETF-QO deficiency. Rocha H, Ferreira R, Carvalho J, Vitorino R, Santa C, Lopes L, Gregersen N, Vilarinho L, Amado F. J Proteomics. 2011 Dec 10;75(1):221-8.

  11. Finding new posttranslational modifications in salivary proline-rich proteins. Vitorino R, Alves R, Barros A, Caseiro A, Ferreira R, Lobo MC, Bastos A, Duarte J, Carvalho D, Santos LL, Amado F. Proteomics Clin Applic. 2011; (5) 3-4: 197 - 197.

  12. Glycation and oxidation of histones H2B and H1: in vitro study and characterization by mass spectrometry.  Guedes S, Vitorino R, Domingues MR, Amado F, Domingues P. Anal Bioanal Chem. 2011 Apr; 399(10):3529-39.

  13. OXPHOS susceptibility to oxidative modifications: The role of heart mitochondrial subcellular location. Padrão AI, Ferreira RM, Vitorino R, Alves RM, Neuparth MJ, Duarte JA, Amado F. Biochim Biophys Acta. 2011 Sep;1807(9):1106-13.

  14. Proteomic evaluation of Escherichia coli isolates from human clinical strains. Pinto L, Poeta P, Radhouani H, Coelho C., Carvalho C, Rodrigues J, Torres C, Vitorino R, Domingues P, Igrejas G. Journal of Integrated OMICS 2011; 1:1 (DOI 10.5584).

  15. Proteomic study in an Escherichia coli strain from seagulls of the Berlengas Natural Reserve of Portugal. Pinto L, Poeta P, Radhouani H, Coelho C., Carvalho C, Rodrigues J, Torres C, Vitorino R, Domingues P, Igrejas G. Journal of Integrated OMICS 2011; 1:1 (DOI 10.5584).

  16. Reactivity of Human Salivary Proteins Families Toward Food Polyphenols.  Soares S, Vitorino R, Oso´rio H, Fernandes A, Vena^ncio A, Mateus N, Amado F, de Freitas V. J Agric Food Chem. 2011 May 25;59(10):5535-5547.

  17. Synthesis and optimization of lectin functionalized nanoprobes for the selective recovery of glycoproteins from human body fluids. Ferreira JA, Daniel-da-Silva AL, Alves RM, Duarte D, Vieira I, Santos LL, Vitorino R, Amado F. Anal Chem. 2011 Sep 15;83(18):7035-43.

  18. Characterization of thymosin ß4 in mammals' saliva. de Sousa-Pereira P, Abrantes J, Colaço B, Castagnola M, Amado F, Esteves PJ, Vitorino R. Peptides. 2012;40C:1-7.

  19. Comparative proteomic map among vanA-containing Enterococcus isolated from yellow-legged gulls. Radhouani H, Poeta P, Pinto L, Monteiro R, Nunes-Miranda J, Correia S, Carvalho C, Rodrigues J, Lopez M, Torres C, Vitorino R, Domingues P, Igrejas G. Journal of Integrated OMICS 2012, 2(1): 46-54(DOI 10.5584).

  20. Effect of lifestyle on age-related mitochondrial protein oxidation in mice cardiac muscle. Padrão AI, Ferreira R, Vitorino R, Alves RM, Figueiredo P, Duarte JA, Amado F. Eur J Appl Physiol 2012 112(4):1467-74.

  21. Effect of lifestyle on age-related mitochondrial protein oxidation in mice cardiac muscle. Padrão AI, Ferreira R, Vitorino R, Alves RM, Figueiredo P, Duarte JA, Amado F. Eur J Appl Physiol. 2012 Apr;112(4):1467-74.

  22. Evaluation of different extraction procedures for salivary peptide analysis. Vitorino R, Barros AS, Caseiro A, Ferreira R, Amado F. Talanta 2012 94:209-15.

  23. High-Yield Expression in Escherichia coli and Purification of Mouse Ubiquitin-Activating Enzyme E1. Carvalho AF, Pinto MP, Grou CP, Vitorino R, Domingues P, Yamao F, Sá-Miranda C, Azevedo JE. Mol Biotechnol. 2012 Jul;51(3):254-61.

  24. Impaired protein quality control system underlies mitochondrial dysfunction in skeletal muscle of streptozotocin-induced diabetic rats. Padrão AI, Carvalho T, Vitorino R, Alves RM, Caseiro A, Duarte JA, Ferreira R, Amado F. Biochim Biophys Acta. 2012 1822(8):1189-1197.

  25. Inability of mutant transrthyretin V30M to cross the bood-eye barrier. Beirão J, Moreira L, Lacerda P, Vitorino R, Beirão I, Torres P, Costa P. Transplantation 2012 94(8):e54-e56.

  26. Protease profiling of different biofluids in type 1 diabetes mellitus. Caseiro A, Ferreira R, Quintaneiro C, Pereira A, Marinheiro R, Vitorino R, Amado F. Clin Biochem 2012 45(18): 1613-9.

  27. Proteome-base biomarkers in Diabetes Mellitus: from discovery to clinical applications. Padrão AI, Ferreira R, Vitorino R, Amado F. Proteomics Clin Applic. 2012; 6(9-10):447-66.

  28. Salivary peptidome in type 1 diabetes mellitus.  Caseiro A, Vitorino R, Barros AS, Ferreira R, Calheiros-Lobo MJ, Carvalho D, Duarte JA, Amado F. Biomed Chromatogr. 2012 May;26(5):571-82.

  29. Spatially distinct mitochondrial populations exhibit different mitofilin levels. Ferreira R, Vitorino R, Padrão AI, Moreira-Gonçalves D, Alves R, Duarte JA, Amado F. Cell Biochemistry & Function 2012 30(5):395-9.

  30. Toward a standardized saliva proteome analysis methodology. Vitorino R, Guedes S, Manadas B, Ferreira R, Amado F. J Proteomics 2012 75(17):5140-65.

  31. An evolutionary perspective of mammal salivary peptide families: Cystatins, histatins, statherin and PRPs. de Sousa-Pereira P, Amado F, Abrantes J, Ferreira R, Esteves PJ, Vitorino R. Arch Oral Biol. 2013;58(5):451-8.

  32. Bladder cancer-induced skeletal muscle wasting: disclosing the role of mitochondria plasticity. Padrão AI, Oliveira P, Vitorino R, Colaço B, Pires MJ, Márquez M, Castellanos E, Neuparth MJ, Teixeira C, Costa C, Moreira-Gonçalves D, Cabral S, Duarte JA, Santos LL, Amado F, Ferreira R. Int J Biochem Cell Biol. (2013) 45(7):1399-1409.

  33. Challenging the limits of detection of sialylated Thomsen-Friedenreich antigens by in-gel deglycosylation and nano-LC-MALDI-TOF-MS. Almeida A, Ferreira JA, Teixeira F, Gomes C, CordeiroMNDS, Osorio H, Santos LL, Reis CA, Vitorino R, Amado F. Electrophoresis. 2013;34:2337-2341.

  34. Characterization of in vitro protein oxidation using mass spectrometry: a time course study of oxidized alpha-amylase. Silva AM, Marçal SL, Vitorino R, Domingues MR, Domingues P. Arch Biochem Biophys. 2013;530(1):23-31.

  35. Characterization of thymosin beta 4 in mammals' saliva. de Sousa-Pereira P, Abrantes J, Colaco B, Castagnola M, Amado F, Esteves PJ, Vitorino R. Peptides. 2013;40:1-7.

  36. Exploring the role of post-translational modifications on protein-protein interactions with survivin. Nogueira-Ferreira R, Vitorino R, Ferreira-Pinto MJ, Ferreira R, Henriques-Coelho T. Arch. Biochem. Biophys. 2013;538:64-70.

  37. Immunoproteomic analysis of Plasmodium falciparum antigens using sera from patients with clinical history of imported malaria. Costa RM, Nogueira F, de Sousa KP, Vitorino R, Silva MS. Malar J. 2013 Mar 18;12:100.

  38. iTRAQ-based Quantitative proteomic analysis of submandibular glands from rats with STZ-induced hyperglycemia. Alves RM, Vitorino R, Padrão AI, Moreira-Gonçalves D, Duarte JA, Ferreira R, Amado F. J Biochem, 2013;153(2):209-20.

  39. Lipidomic characterization of streptozotocin-induced heart mitochondrial dysfunction. Ferreira R, Guerra G, Padrao AI, Melo T, Vitorino R, Duarte JA, Remiao F, Domingues P, Amado F, Domingues MR. Mitochondrion. 2013;13:762-771.

  40. Mitochondria proteome profiling: a comparative analysis between gel- and gel-free approaches. Ferreira R, Rocha H, Almeida V, Padrão AI, Santa C, Vilarinho L, Amado F, Vitorino R. Talanta. 2013;115:277-283.

  41. One decade of salivary proteomics: current approaches and outstanding challenges. Amado F, Ferreira R, Vitorino R. Clin Biochem. 2013;46(6):506-17.

  42. Optimization of Biosurfactant production by Bacillus subtilis strains isolated from Brazilian crude oil and their structural characterization. Pereira J, Gudiña EJ, Costa R, Vitorino R, Rodrigues LR, Teixeira JA, Coutinho JAP. Fuel. 2013 111:259-268.

  43. Overexpression of tumour-associated carbohydrate antigen sialyl-Tn in advanced bladder tumours. Ferreira JA, Videira PA, Lima L, Pereira S, Silva M, Carrascal M, Severino PF, Fernandes E, Almeida A, Costa C Vitorino R, Amaro T, Oliveira MJ, Reis CA, Dall'olio F, Amado F, Santos LL. Mol Oncol. 2013 7(3): 719-31.

  44. Post-translational Modifications and Mass Spectrometry Detection. Silva AMN, Vitorino R, Domingues MRM, Spickett CM, Domingues P. Free Radic. Biol. Med. 2013;65:925-941.
  45. Proteome of the head and thorax salivary glands in the stingless bee Melipona quadrifasciata anthidioides. Elias-Santos D, Fialho MDQ, Vitorino R, Oliveira LL, Zanuncio JC, Serrao JE. Apidologie. 2013;44:684-698.

  46. Remodeling of liver phospholipidomic profile in streptozotocin-induced diabetic rats. Simoes C, Domingues P, Ferreira R, Amado F, Duarte JA, Vitorino R, Neuparth MJ, Nunes C, Rocha C, Duarte I, Domingues MR. Arch. Biochem. Biophys. 2013;538:95-102.

  47. Salivary Proteome and Peptidome Profiling in Type 1 Diabetes Mellitus Using a Quantitative Approach. Caseiro A, Ferreira R, Padrao A, Quintaneiro C, Pereira A, Marinheiro R, Vitorino R, Amado F. J. Proteome Res. 2013;12:1700-1709.

  48. Unraveling the Phosphoproteome Dynamics in Mammal Mitochondria from a Network Perspective. Padrao AI, Vitorino R, Duarte JA, Ferreira R, Arnado F. J. Proteome Res. 2013;12:4257-4267.
última atualização a 24-03-2015
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