microscopy


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microscopy

The Light Microscopy facility (LiM) of the Institute of Biomedicine (iBiMED) supports research on conventional and advanced light microscopy. It is equipped with various microscopes that are available to iBiMED and external researchers.


It also offers advanced services to both intramural and extramural researchers. LiM is integrated in the Portuguese Platform of Bioimaging (PPBI), an infrastructure of the National Roadmap of Research Infrastructures of Strategic Relevance, funded by FCT and the Compete2020 program. The main objectives of the LiM are to provide:
i) advanced microscopy methods for current research in life and health sciences;
ii) training on light microscopy to iBiMED and visiting researchers;
iii) expert advice on experiment design and image analysis;
iv) advanced microscopy seminars and workshops on latest technological advances in light microscopy.


We are committed to the development of light microscopy techniques and their applications to the ongoing projects of iBiMED researchers and also external users. We are currently focused on the following techniques:
1) Continuous development of specialized microscopy techniques and data analysis methods
2) Application of laser scanning confocal microscopy, low-light-level imaging, motion analysis, fixation, labelling, live cells imaging, spectral imaging and linear unmixing, data management, image processing and data analysis, colocalization, deconvolution.

microscopy

Our microscopy platform includes an Olympus IX81 motorized inverted microscope, a Zeiss AxioImager Z1 automated microscope, and a Zeiss LSM 510M confocal microscope equipped with a vast range of high power lasers available from violet to far red (405, 488, 514, 561 and 633 nm excitation wavelengths). The Zeiss LSM 510 META is a confocal laser scanning microscope able to generate high-resolution three-dimensional images of thick specimens. It is an inverted microscope suitable for live cell imaging and photobleaching experiments, equipped with an environmental chamber for temperature-controlled microscopy of living cells. Its META detector can be used for spectral detection and subsequent linear unmixing of overlapping fluorescent stainings, however at a lower sensitivity than its other two PMT detectors.

 
última atualização a 27-03-2019
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